%0 Journal Article
%T The ¦Á-glucosidase inhibiting isoflavones isolated from Belamcanda chinensis leaf extract
%A Chongming Wu
%A Jingyao Shen
%A Pingping He
%A Yan Chen
%J Records of Natural Products
%D 2012
%I ACG Publications
%X The dried rhizome of Belamcanda chinensis is an important Chinese traditional medicine used for the treatment of inflammation and many other disorders. Previously, we reported the hypo- and antihyper-glycemic effects of the aqueous leaf extract of B. chinensis (BCL) and identified the isoflavones as its principal active fraction. In the present study, the ¦Á-glucosidase inhibitory effect of BCL and its rough isoflavone preparation (BIF) was tested in vitro and in vivo. Thirteen isoflavones were isolated from BCL and their ¦Á-glucosidase inhibitory activity was screened in vitro. The results showed that BCL (500 and 1000 mg/kg) and BIF (250 and 500 mg/kg) greatly inhibited the increase in blood glucose level after 5 g/kg starch loading in normal mice. Six out of the thirteen isoflavones (swertisin, 2 ¡± -O-rhamnosylswertisin, genistein, genistin, mangiferin and daidzin) exhibited strong ¦Á-glucosidase inhibitory activity in vitro. HPLC analysis showed that swertisin was the most abundant isoflavone in BCL accounting for 1.24% of BCL, 7.44% of BIF, and 11.24% of the total isoflavone fraction of BCL, respectively. These results demonstrate that BCL possesses significant ¦Á-glucosidase inhibitory activity and swertisin may be the principal active component of BCL in ¦Á-glucosidase inhibition.
%K Belamcanda chinensis
%K diabetes
%K ¦Á-glucosidase inhibitor
%K swertisin
%K isoflavones
%K postprandial hyperglycemia.
%U http://www.acgpubs.org/RNP/2012/Volume%206/Issue%201/16-RNP-1103-563.pdf