%0 Journal Article
%T Study the effect of bacterial 1-aminocyclopropane-1-carboxylate deaminase (ACC deaminase) on resistance to salt stress in tomato plant
%A Maryam SADRNIA
%A Natalia MAKSIMAVA
%A Elena KHROMSOVA
%A Sergei STANISLAVICH
%J Analele Universitatii din Oradea, Fascicula Biologie
%D 2011
%I University of Oradea Publishing House
%X 1-aminocyclopropane-1-carboxylate deaminase (ACC deaminase) produced by rhizobacteria could be remove theethylene precursor and stimulate plant growth. Aim of the work was investigation on effect of rhizosphere bacteria Pseudomonasmendocina containing plasmid carrying gene encoding ACC deaminase on resistance of tomato plant to salinity. Amplification ofacds gene in selected Pseudomonas was performed; the g e n e w a s c l o n e d i n Escherichia coli and was cloned subsequently in P.mendocina. Enzyme activity was determined in cloned Escherichia coli and cloned P. mendocina for confirmation of geneexpression. Effect of bacterial ACC deaminase on resistance of tomato plants to NaCl was studied in Pot and Greenhouse. In potexperiment, tomato plant treated by cloned P. mendocina was compared with plants treated by P. mendocina (without plasmid) andcontrol group. Salinity were established by adding 172 and 207 mM of NaCl to irrigated water. Greenhouse experiments wereconducted in similar groups of bacteria in 207 mM of NaCl. Results obtained from pot experiment revealed that plants treated bycloned P. mendocina in 172 mM of NaCl was showed increasing content of growth than ones treated by P. mendocina and controlas 11%, 18.4% growth for the shoot, 16.6%, 3.7% for roots and 9.6%, 27.5% for wet weight after five weeks, respectively. In 207mM of NaCl, the results were as 14.9 %, 9.7% for shoot, 94.3%, 15.7% for roots and 96.4%, 50.6% for wet weight, respectively. Ingreenhouse experiment, results in same parameter in 207 mM of NaCl were revealed as 63.7%, 7 times for shoot, 2.8, 14 times forroots and 66.1%, 154 times for wet weight, respectively. We concluded that recombinant P. mendocina producing ACC deaminaseby reduction of ethylene content of tomato plant in high salt concentrations could result in improvement of plant resistance tosalinity.
%K Pseudomonas mendocina
%K ACC deaminase
%K ethylene
%K salt stress
%U http://www.bioresearch.ro/bioresearch/2011-2/120-123-AUOFB.18.2.2011%20-%20SADRNIA%20M.%20-%20B.S.Univ.Gen.Belarus%20-%20Study%20the%20effect.pdf