%0 Journal Article
%T Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) supports adhesion and migration of mesenchymal stem cells and tenocytes
%A Alex J Lomas
%A George GQ Chen
%A Alicia J El Haj
%A Nicholas R Forsyth
%J World Journal of Stem Cells
%D 2012
%I Baishideng Publishing Group Co. Limited
%R 10.4252/wjsc.v4.i9.94
%X AIM: To establish the potential of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) as a material for tendon repair. METHODS: The biocompatibility of PHBHHx with both rat tenocytes (rT) and human mesenchymal stem cells (hMSC) was explored by monitoring adhesive characteristics on films of varying weight/volume ratios coupled to a culture atmosphere of either 21% O2 (air) or 2% O2 (physiological normoxia). The diameter and stiffness of PHBHHx films was established using optical coherence tomography and mechanical testing, respectively. RESULTS: Film thickness correlated directly with weight/volume PHBHHx (r2 = 0.9473) ranging from 0.1 mm (0.8% weight/volume) to 0.19 mm (2.4% weight/volume). Film stiffness on the other hand displayed a biphasic response which increased rapidly at values > 1.6% weight/volume. Optimal cell attachment of rT required films of ¡Ý 1.6% and ¡Ý 2.0% weight/volume PHBHHx in 2% O2 and 21% O2 respectively. A qualitative adhesion increase was noted for hMSC in films ¡Ý 1.2% weight/volume, becoming significant at 2% weight/volume in 2% O2. An increase in cell adhesion was also noted with ¡Ý 2% weight/volume PHBHHx in 21% O2. Cell migration into films was not observed. CONCLUSION: This evaluation demonstrates that PHBHHx is a suitable polymer for future cell/polymer replacement strategies in tendon repair.
%K Mesenchymal stem cell
%K Tenocytes
%K Polyhydroxyalkanoates
%K Hypoxia
%K Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)
%U http://www.wjgnet.com/1948-0210/full/v4/i9/94.htm