%0 Journal Article
%T On the activity of alpha-glucanphosphorylase in Setaria pumila and Festuca pratensis
%A Elena Ciornea
%A Gabriela Vasile
%J Analele £¿tiin£¿ifice Ale Universit£¿£¿ii Alexandru Ioan Cuza din Ia£¿i,Sectiunea II A : Genetica si Biologie Moleculara
%D 2008
%I Alexandru Ioan Cuza University of Iasi
%X The main objective of the present study involves determination of the ¦Á-glucanphosphorylase in germinated caryopses of Setaria pumila and Festuca pratensis, through dosing of the anorganic phosphorous, seed germination being performed in Petri plates, at room temperature, for 10 days, while taking over of the samples occurred at intervals of 24 hours. In the enzymatic extracts thus obtained, starch concentration was also determined, by the polarimetric method, while the concentration of total soluble proteins - necessary for the calculation of the specific phosphorolytic activity - was established by the Bradford method. In both graminaceae species, the enzymatic activity was highly reduced in the impregnated seed stage, after which it progressively increases, in parallels with the intensification of starch hydrolysis, for producing the energy and precursors necessary for various metabolic processes.
%U http://www.gbm.bio.uaic.ro/index.php/gbm/article/view/701