%0 Journal Article
%T Accuracy of oxacillin susceptibility testing methods for Staphylococcus aureus
%A Olivia Dorneanu
%A Teodora Vremera
%A Eduard Nastase
%A Catalina Logigan
%J Analele £¿tiin£¿ifice Ale Universit£¿£¿ii Alexandru Ioan Cuza din Ia£¿i,Sectiunea II A : Genetica si Biologie Moleculara
%D 2010
%I Alexandru Ioan Cuza University of Iasi
%X Since oxacillin is the first-choice drug in the treatment of methicillin-susceptible Staphylococcus aureus infections, correct assessment of oxacillin susceptibility is mandatory. METHODS: One hundred and fifty-six S.aureus strains have been used to evaluate the accuracy of disk diffusion method (1 ¦Ìg oxacillin disk and 30 ¦Ìg cefoxitin disk), detection of minimum inhibitory concentration of oxacillin (E-test), and a latex-agglutination test for detection of PBP2a compared with detection of mecA gene by a real-time PCR technique as gold standard. RESULTS: The mecA gene was detected in 68 strains. Sensitivities of the evaluated methods were 98.5%, 100%, and 100%, while specificities were 100% for all three of them. CONCLUSIONS: Accuracy of phenotypic methods for assessment of oxacillin resistance approaches that of detection of mecA for S.aureus isolates
%U http://www.gbm.bio.uaic.ro/index.php/gbm/article/view/754