%0 Journal Article
%T Lysophosphatidic acid induced nuclear translocation of nuclear factor-B in Panc-1 cells by mobilizing cytosolic free calcium
%A Yoshiyuki Arita
%A Tetsuhide Ito
%A Takamasa Oono
%A Ken Kawabe
%A Terumasa Hisano
%A Ryoichi Takayanagi
%J World Journal of Gastroenterology
%D 2008
%I Baishideng Publishing Group Co. Limited
%X AIM: To clarify whether Lysophosphatidic acid (LPA) activates the nuclear translocation of nuclear factor-百B (NF-百B) in pancreatic cancer.METHODS: Panc-1, a human pancreatic cancer cell line, was used throughout the study. The expression of LPA receptors was confirmed by reverse-transcript polymerase chain reaction (RT-PCR). Cytosolic free calcium was measured by fluorescent calcium indicator fura-2, and the localization of NF-百B was visualized by immunofluorescent method with or without various agents, which effect cell signaling.RESULTS: Panc-1 expressed LPA receptors, LPA1, LPA2 and LPA3. LPA caused the elevation of cytosolic free calcium dose-dependently. LPA also caused the nuclear translocation of NF-百B. Cytosolic free calcium was attenuated by pertussis toxin (PTX) and U73122, an inhibitor of phospholipase C. The translocation of NF-百B was similarly attenuated by PTX and U73122, but phorbol ester, an activator of protein kinase C, alone did not translocate NF-百B. Furthermore, the translocation of NF-百B was completely blocked by Ca2+ chelator BAPTA-AM. Thapsigargin, an endoplasmic-reticulum Ca2+-ATPase pump inhibitor, also promoted the translocation of NF-百B. Staurosporine, a protein kinase C inhibitor, attenuated translocation of NF-百B induced by LPA.CONCLUSION: These findings suggest that protein kinase C is activated endogenously in Panc-1, and protein kinase C is essential for activating NF-百B with cytosolic calcium and that LPA induces the nuclear translocation of NF-百B in Panc-1 by mobilizing cytosolic free calcium.
%K Lysophosphatidic acid
%K Nuclear translocation
%K Nuclear factor-百B
%K Cytosolic free calcium
%K Panc-1
%U http://www.wjgnet.com/1007-9327/full/v14/i28/4473.htm