%0 Journal Article
%T Zymomonas mobilis levan production in the presence of antimetabolic angents
%A Cristina Sturzoiu
%A Andrei Tanase
%A Anca Dinischiotu
%A Gheorghe Stoian
%J Analele £¿tiin£¿ifice Ale Universit£¿£¿ii Alexandru Ioan Cuza din Ia£¿i,Sectiunea II A : Genetica si Biologie Moleculara
%D 2010
%I Alexandru Ioan Cuza University of Iasi
%X Our studies have focused on the screening of Zymomonas mobilis bacterial strains capable to produce levan under the action of three antimetabolic agents (sulfafurozol, sulfametaxazol, trimetoprim). The experiments were carried out using spontaneous mutant strains derived from Z.mobilis NCIB 11163 and 11163/70, obtained by supplemented media with methotrexate (600 ¦Ìg/mL) and trimethoprim (1000 ¦Ìg/mL). Exponential growth profiles of bacterial cells and the production of levan were assessed in the absence and presence of antimetabolites of different concentrations. The studies have shown that Z.mobilis 11163/70 strains manifest a progressive growth in the presence of trimethoprim (50 ¦Ìg/mL), an inhibition growth in the presence of sulfametoxazol (100 ¦Ìg/mL). and also good resistance in the presence of sulfafurazol (100 ¦Ìg/mL). Sulphonamides can inhibit the production of levan (Z.mobilis CP4PRRif, Z.mobilis NCIB 11163/70). On the other hand, a stimulation of levan production has been observed in the presence of trimetoprim (50 ¦Ìg/mL) (Z.mobilis CP4Rif and 10988).
%U http://www.gbm.bio.uaic.ro/index.php/gbm/article/view/837