%0 Journal Article
%T PCR-SSCP-DNA sequencing method in detecting PTEN gene mutation and its significance in human gastric cancer
%A Chuan-Yong Guo
%A Xuan-Fu Xu
%A Jian-Ye Wu
%A Shu-Fang Liu
%J World Journal of Gastroenterology
%D 2008
%I Baishideng Publishing Group Co. Limited
%X AIM: To discuss the possible effect of PTEN gene mutations on occurrence and development of gastric cancer.METHODS: Fifty-three gastric cancer specimens were selected to probe PTEN gene mutations in genome of gastric cancer and paracancerous tissues using PCR-SSCP-DNA sequencing method based on microdissection and to observe the protein expression by immunohistochemistry technique.RESULTS: PCR-SSCP-DNA sequencing indicated that 4 kinds of mutation sites were found in 5 of 53 gastric cancer specimens. One kind of mutation was found in exons. AA-TCC mutation was located at 40bp upstream of 3กฏ lateral exon 7 (115946 AA-TCC). Such mutations led to terminator formation in the 297th codon of the PTEN gene. The other 3 kinds of mutation were found in introns, including a G-C point mutation at 91 bp upstream of 5กฏ lateral exon 5(90896 G-C), a T-G point mutation at 24 bp upstream of 5กฏ lateral exon 5 (90963 T-G), and a single base A mutation at 7 bp upstream of 5กฏ lateral exon 5 (90980 A del). The PTEN protein expression in gastric cancer and paracancerous tissues detected using immunohistochemistry technique indicated that the total positive rate of PTEN protein expression was 66% in gastric cancer tissue, which was significantly lower than that (100%) in paracancerous tissues (P < 0.005).CONCLUSION: PTEN gene mutation and expression may play an important role in the occurrence and development of gastric cancer.
%K Gastric cancer
%K PTEN gene
%K PCR-SSCP
%K DNA sequencing
%K Mutation
%U http://www.wjgnet.com/1007-9327/full/v14/i24/3804.htm