%0 Journal Article
%T High expression of osteoglycin decreases gelatinase activity of murine hepatocarcinoma Hca-F cells
%A Xiao-Nan Cui
%A Jian-Wu Tang
%A Bo Song
%A Bo Wang
%A Shan-Yan Chen
%A Li Hou
%J World Journal of Gastroenterology
%D 2009
%I Baishideng Publishing Group Co. Limited
%X AIM: To investigate the possible correlation between osteoglycin expression and gelatinase activity of mouse hepatocarcinoma Hca-F cells.METHODS: A eukaryotic expression plasmid pIRESpuro3 osteoglycin(+) was constructed and transfected into Hca-F cells to investigate the possible correlation between osteoglycin expression and gelatinase activity of Hca-F cells cultured with extract of lymph node, liver, spleen or in DMEM medium. The activity of gelatinases was examined through zymographic analysis.RESULTS: High expression of osteoglycin attenuated the gelatinase activity of Hca-F cells cultured with extract of lymph node, and at the same time, decreased the metastatic potential of Hca-F cells to peripheral lymph nodes in vivo.CONCLUSION: High expression of osteoglycin decreases the gelatinase activity of Hca-F cells cultured with extract of lymph node; regulation of gelatinase activity might be one of mechanisms that osteoglycin contributes to lymphatic metastasis suppression.
%K Osteoglycin
%K Transfection
%K Hepatocellular carcinoma
%K Neoplasm metastasis
%K Genes
%K Gelatinases
%U http://www.wjgnet.com/1007-9327/full/v15/i48/6117.htm