%0 Journal Article
%T Overexpression of polo-like kinase1 predicts a poor prognosis in hepatocellular carcinoma patients
%A Zi-Li He
%A He Zheng
%A Hui Lin
%A Xiong-Ying Miao
%A De-Wu Zhong
%J World Journal of Gastroenterology
%D 2009
%I Baishideng Publishing Group Co. Limited
%X AIM: To elucidate the role of overexpressed polo-like kinase1 (PLK1) in hepatocellular carcinoma (HCC).METHODS: We prospectively collected clinicopathological, immunohistochemical and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) data from 135 HCC patients undergoing successful hepatectomy. The correlations between PLK1 mRNA expression and clinicopathologic variables were analyzed by Mann-Whitney U test. Prognostic factors were identified by univariate and multivariate analyses.RESULTS: Immunohistochemical results showed overexpression of PLK1 was mainly found in tumor tissues compared with tumor-free tissue. A similar mRNA result was obtained by semi-quantitative RT-PCR. A total of 111 samples were positive for PLK1 mRNA expression. The positive expression was correlated with venous invasion, tumor nodules and Edmondson grade. Furthermore, 1, 3, 5-year survival rates in the positive expression group were significantly lower than the negative control group. Multivariate analysis showed that positive PLK1 expression was an independent risk factor for HCC.CONCLUSION: PLK1 could be a potential biomarker for diagnosis and therapy for HCC.
%K Hepatocellular carcinoma
%K Immunohistochemistry
%K Reverse transcription-polymerase chain reaction
%K Survival analysis
%K Polo-like kinase 1
%U http://www.wjgnet.com/1007-9327/full/v15/i33/4177.htm