%0 Journal Article
%T High prevalence of viable Mycobacterium avium subspecies paratuberculosis in Crohn¡¯s disease
%A Juan L Mendoza
%A Amparo San-Pedro
%A Esther Culebras
%A Raquel C¨ªes
%A Carlos Taxonera
%A Raquel Lana
%A Elena Urcelay
%A Fernando de la Torre
%A Juan J Picazo
%A Manuel D¨ªaz-Rubio
%J World Journal of Gastroenterology
%D 2010
%I Baishideng Publishing Group Co. Limited
%X AIM: To examine the detection rate of viable Mycobacterium avium subspecies paratuberculosis (MAP) in patients with inflammatory bowel disease [Crohn¡¯s disease (CD) and ulcerative colitis (UC)].METHODS: Thirty patients with CD (15 with at least one NOD2/CARD15 mutation), 29 with UC, and 10 with no inflammatory bowel disease (IBD). were tested for MAP by polymerase chain reaction (specific IS900 fragment) and blood culture.RESULTS: MAP DNA was detected in all original blood samples and 8-wk blood cultures (CD, UC and non-IBD). Positive MAP DNA status was confirmed by dot blot assays. All 69 cultures were negative by acid-fast Ziehl-Neelsen staining. Viable MAP, in spheroplast form, was isolated from the 18-mo blood cultures of all 30 CD patients, one UC patient, and none of the non-IBD controls. No association was found between positive MAP cultures and use of immunosuppressive drugs or CD-associated single nucleotide polymorphisms.CONCLUSION: MAP is widely present in our area and MAP DNA can be recovered from the blood of CD, UC and non-IBD patients. However, MAP spheroplasts were only found in CD patients.
%K Mycobacterium avium subspecies paratuberculosis
%K Crohn¡¯s disease
%K Ulcerative colitis
%K Inflammatory bowel disease
%K Polymerase chain reaction
%K Genetic susceptibility
%U http://www.wjgnet.com/1007-9327/full/v16/i36/4558.htm