%0 Journal Article
%T Butyrate stimulates IL-32¦Á expression in human intestinal epithelial cell lines
%A Ayako Kobori
%A Shigeki Bamba
%A Hirotsugu Imaeda
%A Hiromitsu Ban
%A Tomoyuki Tsujikawa
%A Yasuharu Saito
%A Yoshihide Fujiyama
%A Akira Andoh
%J World Journal of Gastroenterology
%D 2010
%I Baishideng Publishing Group Co. Limited
%X AIM: To investigate the effects of butyrate on interleukin (IL)-32¦Á expression in epithelial cell lines.METHODS: The human intestinal epithelial cell lines HT-29, SW480, and T84 were used. Intracellular IL-32¦Á was determined by Western blotting analyses. IL-32¦Á mRNA expression was analyzed by real-time polymerase chain reaction.RESULTS: Acetate and propionate had no effects on IL-32¦Á mRNA expression. Butyrate significantly enhanced IL-32¦Á expression in all cell lines. Butyrate also up-regulated IL-1¦Â-induced IL-32¦Á mRNA expression. Butyrate did not modulate the activation of phosphatidylinositol 3-kinase (PI3K), a mediator of IL-32¦Á expression. Like butyrate, trichostatin A, a histone deacetylase inhibitor, also enhanced IL-1¦Â-induced IL-32¦Á mRNA expression.CONCLUSION: Butyrate stimulated IL-32¦Á expression in epithelial cell lines. An epigenetic mechanism, such as histone hyperacetylation, might be involved in the action of butyrate on IL-32¦Á expression.
%K Cytokine
%K Histone acetylation
%K Inflammatory bowel disease
%U http://www.wjgnet.com/1007-9327/full/v16/i19/2355.htm