%0 Journal Article
%T Plasma miR-216a as a potential marker of pancreatic injury in a rat model of acute pancreatitis
%A Xiang-Yu Kong
%A Yi-Qi Du
%A Lei Li
%A Jian-Qiang Liu
%A Guo-Kun Wang
%A Jia-Qi Zhu
%A Xiao-Hua Man
%A Yan-Fang Gong
%A Li-Ning Xiao
%A Yong-Zhi Zheng
%A Shang-Xin Deng
%A Jun-Jun Gu
%A Zhao-Shen Li
%J World Journal of Gastroenterology
%D 2010
%I Baishideng Publishing Group Co. Limited
%X AIM: To study the potential value and specificity of plasma miR-216a as a marker for pancreatic injury.METHODS: Two rat models were applied in this article: L-arginine-induced acute pancreatitis was used as one model to explore the potential value of plasma miR-216a for detection of pancreatic injury; nonlethal sepsis induced in rats by single puncture cecal ligation and puncture (CLP) was used as the other model to evaluate the specificity of plasma miR-216a compared with two commonly used markers (amylase and lipase) for acute pancreatitis. Plasmas were sampled from rats at indicated time points and total RNA was isolated. Real-Time Quantitative reverse transcriptase-polymerase chain reaction was used to quantify miR-216a in plasmas.RESULTS: In the acute pancreatitis model, among five time points at which plasmas were sampled, miR-216a concentrations were significantly elevated 24 h after arginine administration and remained significantly increased until 48 h after operation (compared with 0 h time point, P < 0.01, Kruskal-Wallis Test). In the CLP model, plasma amylase and lipase, two commonly used biomarkers for acute pancreatitis, were significantly elevated 24 h after operation (compared with 0 h time point, P < 0.01 and 0.05 respectively, Pairwise Bonferroni corrected t-tests), while miR-216a remained undetectable among four tested time points.CONCLUSION: Our article showed for the first time that plasma miR-216a might serve as a candidate marker of pancreatic injury with novel specificity.
%K MiR-216a
%K Plasma miRNA
%K Pancreatic injury
%K Acute pancreatitis
%K Biomarker
%U http://www.wjgnet.com/1007-9327/full/v16/i36/4599.htm