%0 Journal Article
%T Study of Physiological Potentiality of Callus and Plant Regeneration through Somatic Embryogenesis in Rice Variety Swarna
%A Sananda Mondal
%A R.P. Singh
%A Chaitali Sen
%A Bandana Bose
%J American Journal of Plant Physiology
%D 2011
%I Science publications
%X The regeneration protocol was developed from dehusked and sterilized seeds of rice variety Swarna (MTU 7029) to study callus induction, callus growth rate and regeneration potentiality. Different ranges of 2,4-D (1-4 mg L-1) and combinations of 6-Benzyl Aminopurine (BAP) (2-4 mg L-1) and a-Naphthalene Acetic Acid (NAA) (1 mg L-1) were used for callus induction, somatic embryo formation and plantlets regeneration respectively. Ninty six percent callus induction efficacy was found with 4 mg L-1 2,4-D. Whereas the high frequency of plantlets regenerated from embryogenic calli were observed in the combination of 4 mg L-1 BAP and 1 mg L-1 NAA; the embryogenic calli obtained from 2 mg L-1 2,4-D concentration performed better in this media (4 mg L-1 BAP and 1 mg L-1 NAA) than the other three used concentrations of 2,4-D. Physiological potentiality of 45 days old calli in terms of fresh and dry weights and proline content were showed a positive correlation. However, 4 mg L-1 2,4-D in medium showed low regeneration capacity of calli.
%K Explant
%K physiological potentiality
%K Oryza sativa
%K proline
%K dehusked seed
%U http://docsdrive.com/pdfs/academicjournals/ajpp/2011/91-98.pdf