%0 Journal Article
%T Nuevos Perfiles Gen谷ticos de Salmonella Enteritidis identificados en Luj芍n, Argentina New Genetic Profiles of Salmonella Enteritidis identified in Lujan, Argentina
%A Ricardo J Anselmo
%A Hebe A Barrios
%J Informaci車n Tecnol車gica
%D 2012
%I Centro de Informaci車n Tecnol車gica
%X El objetivo del presente trabajo fue aislar y seleccionar bacteri車fagos salvajes de aguas del r赤o Luj芍n en Argentina, contra Salmonella Enteritidis, fagotipificar 86 cepas de esta serovariedad y evaluar los perfiles gen谷ticos de representantes de los grupos de especificidad surgidos. La concentraci車n de bacteri車fagos de agua de r赤o se realiz車 mediante el hisopo de Moore con 60 minutos de exposici車n a la corriente de agua. El aislamiento y purificaci車n se efectu車 por la t谷cnica de doble capa de agar. Al fagotipificar los 86 aislamientos por metodolog赤a estandarizada surgieron cuatro grupos de especificidad. La subtipificaci車n por electroforesis en campo pulsado realizada a un representante de cada grupo, estableci車 que dos grupos correspondieron al fagotipo PT4, predominante en Latinoam谷rica. Los dos grupos restantes presentaron dos perfiles gen谷ticos que no se hab赤an encontrado antes en la base de datos de la Red PulseNet de Am谷rica Latina y el Caribe. The aim of this study was isolating and selecting wild bacteriophages from waters of the Lujan river in Argentina, against Salmonella Enteritidis, phage typing 86 strains of this serovariety and evaluating the genetic profiles of samples from the emerged specific groups. Bacteriophages concentration of river water was performed using the Moore swab with 60 minutes of exposure to water flow. Isolation and purification was carried out by the technique of double-layer agar. Eighty six isolates were phage-typed by standardized methodology and four groups of specificity appeared. Subtyping by pulsed field gel electrophoresis conducted on one sample of each group, establishing that two phage group corresponded to PT4 phagotype, which is the most common in Latin America. The other two groups had two genetic profiles that were not previously found in the database Latin America and the Caribbean PulseNet Network
%K perfiles gen谷ticos
%K Salmonella Enteritidis
%K bacteri車fagos salvajes
%K fagotipificaci車n
%K genetic profiles
%K Salmonella Enteritidis
%K wild bacteriophages
%K phage typing
%U http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-07642012000300011