%0 Journal Article
%T Effects of ethanol on the properties of platelets and endothelial cells in model experiments
%A Ksenija Stach
%A Anna-Isabelle K£¿lsch
%A Christel Wei£¿
%A Elif Elmas
%J World Journal of Cardiology
%D 2012
%I Baishideng Publishing Group Co. Limited
%R 10.4330/wjc.v4.i6.201
%X AIM: To investigate effects of ethanol on activity markers of atherosclerosis in an in vitro endothelial cell model. METHODS: After 24 h incubation with ethanol (0.0095%), human umbilical vein endothelial cells were stimulated for 1 h with lipopolysaccharide, and were then incubated in direct contact with activated platelets. Following this incubation, the expression of CD40L and CD62P on platelets, and the expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), urokinase plasminogen activator receptor (uPAR), and membrane-type 1 matrix metalloproteinase (MT1-MMP) on endothelial cells were measured by flow cytometry. RESULTS: The increased expression of VCAM-1 and uPAR on endothelial cells by proinflammatory stimulation with activated platelets was significantly reduced through pre-incubation with ethanol (P < 0.05). Furthermore, platelets in direct contact with ethanol and with endothelial cells pre-incubated in ethanol showed a significant reduction in their CD40L expression (P < 0.05). Ethanol had no significant effect on ICAM-1 and MT1-MMP expression on endothelial cells. CONCLUSION: Ethanol directly attenuates platelet activation and has significant endothelial cell-mediated effects on selected markers of atherosclerosis in vitro. These findings underline possible protective effects of ethanol on atherosclerosis.
%K Platelets
%K Endothelial cells
%K Ethanol
%K Inflammation
%K Atherosclerosis
%U http://www.wjgnet.com/1949-8462/full/v4/i6/201.htm