%0 Journal Article
%T Modulation of heat shock protein response in SH-SY5Y by mobile phone microwaves
%A Emanuele Calabr¨°
%A Salvatore Condello
%A Monica Curr¨°
%A Nadia Ferlazzo
%J World Journal of Biological Chemistry
%D 2012
%I Baishideng Publishing Group Co. Limited
%R 10.4331/wjbc.v3.i2.34
%X AIM: To investigate putative biological damage caused by GSM mobile phone frequencies by assessing electromagnetic fields during mobile phone working. METHODS: Neuron-like cells, obtained by retinoic-acid-induced differentiation of human neuroblastoma SH-SY5Y cells, were exposed for 2 h and 4 h to microwaves at 1800 MHz frequency bands. RESULTS: Cell stress response was evaluated by MTT assay as well as changes in the heat shock protein expression (Hsp20, Hsp27 and Hsp70) and caspase-3 activity levels, as biomarkers of apoptotic pathway. Under our experimental conditions, neither cell viability nor Hsp27 expression nor caspase-3 activity was significantly changed. Interestingly, a significant decrease in Hsp20 expression was observed at both times of exposure, whereas Hsp70 levels were significantly increased only after 4 h exposure. CONCLUSION: The modulation of the expression of Hsps in neuronal cells can be an early response to radiofrequency microwaves.
%K Apoptosis
%K Electromagnetic field
%K Heat shock proteins
%K Microwaves
%K SH-SY5Y differentiated cells
%U http://www.wjgnet.com/1949-8454/full/v3/i2/34.htm