%0 Journal Article
%T Factors Affecting the Biomass and Lipid Production from Chlorella sp. TISTR 8990 under Mixotrophic Culture
%A Mohammad Ariful HAQUE
%A Phuwadol BANGRAK
%A Sarote SIRISANSANEEYAKUL
%A Wanna CHOORIT
%J Walailak Journal of Science and Technology
%D 2012
%I Walailak University
%R 10.2004/vol10iss1ppaccepted manuscript
%X Effects of media compositions on biomass and lipid accumulation of the isolate Chlorella sp. TISTR 8990 were investigated under a Plackett-Burman experimental design with mixotrophic cultivation conditions. Under this experimental design there were 15 different runs with ten factors-yeast extract, KH2PO4, MgSO4, FeSO4, MnCl2, CuSO4, Na2MoO4, H3BO3, ZnSO4 and pH. Cultures were grown mixotrophically under 16 h light and 8 h dark regime at 30 oC for a period of 7 days. During the light regime, the light intensity at the surface of the vessels and agitation speed were set to 67.5 ¦̀mol photons m-2s-1 and 150 rpm, respectively. Initial cell concentration was set to an absorbance (A540) of 0.5. For high biomass production (2.2 g/L, run no. 6), the most effective and significant factors were yeast extract, KH2PO4, FeSO4 and ZnSO4 at concentrations 0.3 g/L, 0.3 g/L, 3 mg/L and 0.3 mg/L, respectively. Whereas for high lipid accumulation (19.59 %DCW, run no. 2), these were KH2PO4, pH and yeast extract, at a level of 1.7 g/L, 6.0 and 0.1 g/L, respectively. No significant factors were obtained for higher lipid content. The best treatment for biomass and lipid content was run no. 6, whose medium formula consisted of 0.3 g/L yeast extract, 1.7 g/L KH2PO4, 1.7 g/L MgSO4, 1 mg/L FeSO4, 0.9 mg/L MnCl2, and pH 7.0, together with fixed concentrations of glucose, NaHCO3 and KNO3 at 5 g/L, 0.05 g/L and 0.5 g/L, respectively.
%K Biomass
%K Chlorella sp.
%K mixotroph
%K Plackett-Burman design
%K single cell oils
%U http://wjst.wu.ac.th/index.php/wjst/article/view/454