%0 Journal Article
%T Detection of AmpC Beta-lactamases among Escherichia coli isolates at a tertiary care hospital in Karnataka
%A Smitha O. Bagali
%A B.V. Peerapur
%J Al Ameen Journal of Medical Sciences
%D 2013
%I Dr.B.S.Patil
%X Background & objective: AmpC ¦Â-lactamases are clinically significant because they may confer resistance to a wide variety of ¦Â-lactam drugs, including ¦Á-methoxy-¦Â-lactams, such as cefoxitin, narrow-, expanded- and broad-spectrum cephalosporins, ¦Â-lactam-¦Â-lactamase inhibitor combinations and aztreonam. Although reported with increasing frequency the true occurrence in different organisms remains unknown. The present study was conducted to determine the occurrence of AmpC ¦Â-lactamases among the clinical isolates of Escherichia coli. Methods: A total of 100 non-repeat clinical isolates obtained from urine, pus, sputum, blood and body fluids were taken. All the isolates were screened for AmpC ¦Â-lactamases by standard disc diffusion breakpoint for cefoxitin (30¦Ìg). Isolates with zone diameter less than 18 mm were tested for AmpC activity by AmpC disc test. Results: Of the 100 isolates that were tested, 30 yielded cefoxitin zone diameters less than 18 mm (screen positive). Production of AmpC ¦Â-lactamase was detected in 24 isolates by AmpC disc test. Conclusion: AmpC disc test can be used as a simple, convenient and rapid screening test for detection of AmpC ¦Â lactamase in clinical laboratories.
%K AmpC ¦Â-lactamase
%K AmpC disc test
%K Cefoxitin
%U http://ajms.alameenmedical.org/ArticlePDFs%5CAJMS%20V6.N1.2013%20p%2085-87.pdf