%0 Journal Article %T LACTIC ACID PRODUCTION VIA CASSAVA-FLOURHYDROLYSATE FERMENTATION PRODUCCI¨®N DE ¨¢CIDO L¨¢CTICO VIA FERMENTATIVA A PARTIR DE HIDROLIZADO DE HARINA DE YUCA %A Joan E. QUINTERO M. %A Alejandro ACOSTA C. %A Carlos MEJ¨ªA G %A Rigoberto R¨ªOS E. %J Vitae %D 2012 %I Universidad de Antioquia %X Background: Lactic acid (LA) is a carboxylic acid widely used as preservative, acidulant, and/or flavouring in food industry; it is also used as a raw material for the production of lactate ester, propylene glycol, 2,3-pentanedione, propanoic acid, acrylic acid and acetaldehyde. In recent years, the demand for LA production has dramatically increased due to its application as a monomer for poly-lactic acid synthesis, a biodegradable polymer used as a plastic in many industrial applications. LA can be produced either by fermentation or chemical synthesis; the former route has received considerable interest, due to environmental concerns and the limited nature of petrochemical feedstocks; thus, 90% of LA produced worldwide is obtained by fermentation, this process comprises the bioconversion of a sugar solution (carbohydrates) into LA in the presence of a microorganism. Objectives: This work is aimed at studying the effect of pH control and culture media composition on the LA production using renewable sources from the agroindustry sector. Methods: A Lactobacillus brevis strain is used to perform lab scale experiments under aerobic and anaerobic conditions, using three different culture media compositions: a high nutritional content medium (MRS), as a reference, a low nutritional content medium with glucose as the only carbon source (GM), and a potential low nutritional content medium with cassava flour as carbon source (HY1). Results: The higher LA production is accomplished under anaerobic conditions, 17.6 ¡À 0.1, 12.6 ¡À 0.2 y 13.6 ¡À 0.2 g LA/L, for MRS, GM and HY1 medium, respectively. The effect of pH on LA biosynthesis in a 5L bioreactor is also studied using the HY1 medium. For a fermentation time of 120 h, the highest LA concentration obtained was 24.3 ¡À 0.7g LA/L, productivity 0.20 g/L/h, Y P/S 0.32g LA/g syrup, at pH 6.5. Conclusions: These results are comparable with those using expensive carbon sources such as glucose, and show cassava flour as a promising low-cost substrate source for lab and eventually large scale LA biosynthesis. Antecedentes: El ¨¢cido l¨¢ctico (AL) es un ¨¢cido carbox¨ªlico utilizado en la industria alimentaria como conservante, acidulante y saborizante; tambi¨¦n es usado como materia prima para la producci¨®n de ¨¦ster de lactato, propilenglicol, 2,3-pentanodiona, ¨¢cido propanoico, ¨¢cido acr¨ªlico y acetaldeh¨ªdo. La demanda de AL ha aumentado debido a su aplicaci¨®n como mon¨®mero en la s¨ªntesis de ¨¢cido poli-l¨¢ctico, un pol¨ªmero biodegradable usado como pl¨¢stico en aplicaciones industriales. El AL puede ser producido por fermentaci¨® %K ¨¢cido l¨¢ctico %K jarabe de yuca %K sustratos econ¨®micos %K Lactobacillus brevis %K efecto del pH %K Lactic acid %K cassava flour %K cassava waste material %K Lactobacillus brevis %K pH effect %U http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0121-40042012000300007