%0 Journal Article %T Mapping protein–RNA interactions %A Vaughan R %A Running W %A Qi R %A Kao CC %J Virus Adaptation and Treatment %D 2012 %I Dove Medical Press %X Robert Vaughan, William Running, Rongsu Qi, C Cheng KaoThe Biochemistry Interdisciplinary Program, Indiana University, Bloomington, IN, USAAbstract: There is a significant need to develop approaches for rapid and accurate mapping of protein¨Cribonucleic acid (RNA) interactions, especially to complement structure-based methods. Approaches using mass spectrometry to map regions in proteins that contact RNA have now been established. These include a reversible crosslinking affinity purification method, residue-specific modification interference assay, and photoactivatable crosslinking and mass spectrometry. Novel methods to identify nucleotides within RNA that contact proteins using photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation are also available. In combination, these methods should generate results that will lead to more specific hypotheses concerning the biological properties of protein¨CRNA interactions. This review summarizes some recent advances in select assays useful for mapping protein¨CRNA interactions.Keywords: hepatitis C virus, positive-strand RNA virus, reversible crosslinking, RNA binding, mass spectrometry %U http://www.dovepress.com/mapping-proteinndashrna-interactions-a9802