%0 Journal Article
%T Glycogen Synthase Kinase-3 regulates IGFBP-1 gene transcription through the Thymine-rich Insulin Response Element
%A David Finlay
%A Satish Patel
%A Lorna M Dickson
%A Natalia Shpiro
%A Rodolfo Marquez
%A Chris J Rhodes
%A Calum Sutherland
%J BMC Molecular Biology
%D 2004
%I BioMed Central
%R 10.1186/1471-2199-5-15
%X In this report we demonstrate that in H4IIE-C3 cells, four distinct classes of GSK-3 inhibitor mimic the effect of insulin on a third TIRE-containing gene, IGFBP-1. We identify the TIRE as the minimum requirement for inhibition by these agents, and demonstrate that the target of GSK-3 is unlikely to be the postulated TIRE-binding protein FOXO-1. Importantly, overexpression of GSK-3 in cells reduces the insulin regulation of TIRE activity as well as endogenous IGFBP-1 expression.These results implicate GSK-3 as an intermediate in the pathway from the insulin receptor to the TIRE. Indeed, this is the first demonstration of an absolute requirement for GSK-3 inhibition in insulin regulation of gene transcription. These data support the potential use of GSK-3 inhibitors in the treatment of insulin resistant states such as Type 2 diabetes mellitus, but suggest that it will be important to identify all TIRE-containing genes to assess potential side effects of these agents.Insulin-like growth factors (IGF-I and II) have a broad range of biological activities that include the stimulation of mitogenesis and differentiation, and insulin-like effects on glucose uptake and lipogenesis [1]. These activities are modulated by a family of six binding proteins, termed the IGF-binding proteins (IGFBPs 1¨C6) that bind IGF-I and IGF-II with high affinity (for review see [2]). IGFBP-1 binds and inhibits the activity of IGF-I and IGF-II in plasma, by regulating their bioavailability [3]. Administration of excess IGFBP-1, or overexpression of IGFBP-1 in transgenic mice, leads to glucose intolerance and hyperinsulinaemia [4,5]. Meanwhile, IGFBP-1 expression can be dynamically regulated by nutritional status, increasing during fasting, malnutrition and diabetes but decreasing upon re-feeding or insulin treatment [6-8]. Hepatic IGFBP-1 gene transcription is rapidly and completely inhibited by insulin [9,10], however, the signalling pathway(s) that mediates this effect is less well defined. Ins
%K GSK-3
%K Insulin
%K IGFBP-1 gene transcription
%K TIRE
%K CHIR99021
%U http://www.biomedcentral.com/1471-2199/5/15