%0 Journal Article
%T TAF6¦Ä orchestrates an apoptotic transcriptome profile and interacts functionally with p53
%A Emmanuelle Wilhelm
%A Mara Kornete
%A Brice Targat
%A Jimmy Vigneault-Edwards
%A Mattia Frontini
%A Laszlo Tora
%A Arndt Benecke
%A Brendan Bell
%J BMC Molecular Biology
%D 2010
%I BioMed Central
%R 10.1186/1471-2199-11-10
%X Here we define the impact of endogenous TAF6¦Ä expression on the global transcriptome landscape. TAF6¦Ä was found to orchestrate a transcription profile that included statistically significant enrichment of genes of apoptotic function. Interestingly, gene expression patterns controlled by TAF6¦Ä share similarities with, but are not equivalent to, those reported to change following TAF9 and/or TAF9b depletion. Finally, because TAF6¦Ä regulates certain p53 target genes, we tested and demonstrated a physical and functional interaction between TAF6¦Ä and p53.Together our data define a TAF6¦Ä-driven apoptotic gene expression program and show crosstalk between the p53 and TAF6¦Ä pathways.Apoptosis is an active program of cell death that is required for normal development and tissue homeostasis in metazoans [1]. The deregulation of apoptotic pathways underlies many human diseases [2]. Consequently, apoptotic pathways represent potential targets for therapeutic control of cell death for diseases including neurodegenerative disorders, autoimmune diseases and cancer [3]. Our previous studies have uncovered the existence of an apoptotic pathway termed the TAF6¦Ä pathway that controls cell death [4,5].TAF6¦Ä is an inducible splice variant of the TFIID subunit TAF6 (previously termed hTAFII70 or hTAFII80). TFIID is a multiprotein complex containing the TATA-binding protein (TBP) and up to 14 evolutionarily conserved TBP-associated factors (TAFs) [6,7]. TFIID is the primary core promoter recognition complex for RNA polymerase II (pol II) and thus plays a key role in the regulation of transcription of protein-coding genes [8]. The major TAF6¦Á isoform is ubiquitously expressed [9] whereas strong expression of the TAF6¦Ä isoform has only been detected in apoptotic conditions (e.g. HL-60 cells undergoing retinoic acid dependent death) [4]. The use of modified antisense RNA oligonucleotides, also termed splice-switching oligonucleotides (SSO), to experimentally direct the expression of endogeno
%U http://www.biomedcentral.com/1471-2199/11/10