%0 Journal Article
%T Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil
%A YUDHIE ISTIANTO
%A RADEN SETYO ADJI KOESOEMOWIDODO
%A YOSHIO WATANABE
%A HARDANING PRANAMUDA
%J Microbiology Indonesia
%D 2012
%I Indonesian Society for Microbiology
%R 10.5454/mi.6.1.7
%X Phenol treatment was applied for isolation of rare Actinomycetes using 25 soil samples collected from Pulau Seribu, Tanjung Redep, Manokwari, and Halmahera. The samples were air-dried and suspended in 1.5 % (w/v) phenol solution at 30 oC for 30 minutes, and subsequently cultured on plates of humic acid-vitamin agar (HVA) medium supplemented with cycloheximide (50 ¦Ìg/mL) and nystatin (50 ¦Ìg/mL). A total of 61 isolates were obtained and the most dominant isolates were not Streptomyces (only 24.6%), whereas other genera such as Micromonospora, Actinomadura, Microbispora and Polymorphospora were isolated with ratios of 49.2%, 13.1%, 9.8%, and 3.3%, respectively.
%K phenol method
%K rare-Actinomycetes
%K selective isolation method
%K soil sample
%U http://jurnal.permi.or.id/index.php/mionline/article/view/163