%0 Journal Article
%T Isolation of bifidobacteria for blood group secretor status targeted personalised nutrition
%A Harri M£¿kivuokko
%A Pirjo Wacklin
%A Marjorie E. Koenen
%A Karoliina Laamanen
%J Microbial Ecology in Health and Disease
%D 2012
%I Co-Action Publishing
%R 10.3402/mehd.v23i0.18578
%X Background: Currently, there is a constant need to find microbial products for maintaining or even improving host microbiota balance that could be targeted to a selected consumer group. Blood group secretor status, determining the ABO status, could be used to stratify the consumer group. Objective: We have applied a validated upper intestinal tract model (TIM-1) and culturing methods to screen potential probiotic bacteria from faeces of blood secretor and non-secretor individuals. Design: Faecal samples from healthy volunteers were pooled to age- and sex-matched secretor and non-secretor pools. Faecal pools were run through separate TIM-1 simulations, and bacteria were cultivated from samples taken at different stages of simulations for characterisation. Results: Microbes in secretor pool survived the transit through TIM-1 system better than microbes of non-secretor pool, especially bifidobacteria and anaerobes were highly affected. The differences in numbers of bifidobacteria and lactobacilli isolates after plate cultivations and further the number of distinct RAPD-genotypes was clearly lower in non-secretor pool than in secretor pool. Conclusions: In the present study, we showed that microbiota of secretor and non-secretor individuals tolerate gastrointestinal conditions differently and that a combination of gastrointestinal simulations and cultivation methods proved to be a promising tool for isolating potentially probiotic bacteria.
%K gastrointestinal simulation
%K probiotic screening
%K Bifidobacterium
%K intestinal
%K ABO blood group
%K secretor
%K non-secretor
%U http://www.microbecolhealthdis.net/index.php/mehd/article/view/18578/22515