%0 Journal Article
%T Isolation and Identification of a Thermostable Amylase-Producing Bacterium from Hatuasa Hotspring
%A DOMINGGUS MALLE
%A JUNUS PICARIMA
%A LAURY CHARA HUWAE
%A INDRA RAHMAWATI
%J Microbiology Indonesia
%D 2012
%I Indonesian Society for Microbiology
%R 10.5454/mi.6.2.5
%X Hot springs are a common source of thermophyles which produce thermostable enzymes. The objective of this study was to isolate and identify thermostable amylase-producing bacteria from a local geothermal spring. An amylase¨Cproducing bacterium strain was isolated from this hot spring which excreted amylase after being grown on starch agar screening plates at 37 ¡ãC. It was identified as Bacillus megaterium using the method of 16S ribosomal DNA. The organism is a rod-shape and is a spore-forming bacterium. Maximum amylase production was achieved after incubation in the production media for 72 hours. Preliminary analysis of the secreted amylase showed that the enzyme could bind to DEAE-Sepharose matrix and was discharged by eluting with 0.5 M NaCl. The partially purified enzyme was stable up to 75 ¡ãC, showing that this enzyme might have potential application in the starch-processing industry.
%K Bacillus
%K hotsprings
%K isolation
%K thermostable amylase
%U http://jurnal.permi.or.id/index.php/mionline/article/view/165