%0 Journal Article
%T Viability of Bifidobacterium Pseudocatenulatum G4 after Spray-Drying and Freeze-Drying
%A Stephenie Wong
%A Barka Mohammed Kabeir
%A Shuhaimi Mustafa
%A Rosfarizan Mohamad
%J Microbiology Insights
%D 2010
%I 
%X Viability of Bifidobacterium pseudocatenulatum G4 following spray-drying and freeze-drying in skim milk was evaluated. After spray-drying, the strain experienced over 99% loss in viability regardless of the air outlet temperature (75 and 85 ¡ãC) and the heat-adaptation temperature (45 and 65 ¡ãC, 30 min). The use of heat-adaptation treatment to improve the thermotolerance of this strain was ineffective. On the other hand, the strain showed a superior survival at 71.65%¨C82.07% after freeze-drying. Viable populations of 9.319¨C9.487 log10 cfu/g were obtained when different combinations of skim milk and sugar were used as cryoprotectant. However, the addition of sugars did not result in increased survival during the freeze-drying process. Hence, 10% (w/v) skim milk alone is recommended as a suitable protectant and drying medium for this strain. The residual moisture content obtained was 4.41% ¡À 0.44%.
%U http://la-press.com/viability-of-bifidobacterium-pseudocatenulatum-g4-after-spray-drying-a-a2008