%0 Journal Article
%T Characterization of the mrgRS locus of the opportunistic pathogen Burkholderia pseudomallei: temperature regulates the expression of a two-component signal transduction system
%A Magdy E Mahfouz
%A T Hilton Grayson
%A David AB Dance
%A Martyn L Gilpin
%J BMC Microbiology
%D 2006
%I BioMed Central
%R 10.1186/1471-2180-6-70
%X The locus was present and expressed in a variety of B. pseudomallei human and environmental isolates but was absent from other Burkholderia species, B. cepacia, B. cocovenenans, B. plantarii, B. thailandensis, B. vandii, and B. vietnamiensis. A 2128 bp sequence, including the full response regulator mrgR, but not the sensor kinase mrgS, was present in the B. mallei genome. Restriction fragment length polymorphism downstream from mrgRS showed two distinct groups were present among B. pseudomallei isolates. Our analysis of the open reading frames in this region of the genome revealed that transposase and bacteriophage activity may help explain this variation. MrgR and MrgS proteins were expressed in B. pseudomallei 204 cultured at different pH, salinity and temperatures and the expression was substantially reduced at 25ˇăC compared with 37ˇăC or 42ˇăC but was mostly unaffected by pH or salinity, although at 25ˇăC and 0.15% NaCl a small increase in MrgR expression was observed at pH 5. MrgR was recognized by antibodies in convalescent sera pooled from melioidosis patients.The results suggest that mrgRS regulates an adaptive response to temperature that may be essential for pathogenesis, particularly during the initial phases of infection. B. pseudomallei and B. mallei are very closely related species that differ in their capacity to adapt to changing environmental conditions. Modifications in this region of the genome may assist our understanding of the reasons for this difference.The saprophyte Burkholderia pseudomallei is an opportunistic pathogen that is capable of intracellular survival [1,2] and causes melioidosis, a frequently fatal disease of humans and animals, which can be difficult to diagnose [3]. Although the pathogen is mainly distributed in the soil and water of tropical regions, especially south-east Asia and northern Australia, it is highly adaptable, nutritionally versatile and able to survive and grow in a wide range of environments [4-8]. The disease enc
%U http://www.biomedcentral.com/1471-2180/6/70