%0 Journal Article
%T Gene expression profile and pathogenicity of biofilm-forming Prevotella intermedia strain 17
%A Takeshi Yamanaka
%A Tomoyo Furukawa
%A Chiho Matsumoto-Mashimo
%A Kazuyoshi Yamane
%A Chieko Sugimori
%A Takayuki Nambu
%A Naoki Mori
%A Hiroyuki Nishikawa
%A Clay B Walker
%A Kai-Poon Leung
%A Hisanori Fukushima
%J BMC Microbiology
%D 2009
%I BioMed Central
%R 10.1186/1471-2180-9-11
%X Strain 17 induced greater abscess formation in mice as compared to that of the variant. Strain 17, but not 17-2 showed an ability to interfere with the phagocytic activity of human neutrophils. Expression of several genes which including those for heat shock proteins (DnaJ, DnaK, ClpB, GroEL and GroES) were up-regulated two to four-fold with statistical significance in biofilm-forming strain 17 as compared to the variant strain 17-2. Strain 17 in solid culture condition exhibited more than eight-fold up-regulated expression levels of several genes which including those for levanase, extracytoplasmic function-subfamily sigma factor (¦ÒE; putative) and polysialic acid transport protein (KpsD), as compared to those of strain 17 in liquid culture media.These results demonstrate that the capacity to form biofilm in P. intermedia contribute to their resistance against host innate defence responses.Prevotella intermedia, a gram-negative, black-pigmented anaerobic rod, is frequently isolated from periodontal pockets of patients with chronic periodontitis [1], acute necrotizing ulcerative gingivitis [2], pregnancy gingivitis [3], and endodontic lesions [4-6]. This organism possesses a number of virulent factors that underlie it's pathogenic potential for causing infections [7-11].P. intermedia strain 17 was initially isolated from a chronic periodontitis lesion in our laboratory [12] and some of its phenotypic characteristics were determined. Among these included the ability of the organism to: (a) produce viscous materials in vitro [12]; (b) invade human oral epithelial cells [13]; and (c) stimulate CD4+ T cells expressing V¦Â8, V¦Â12 and V¦Â17 [14]. More recently, the whole genome sequence of strain 17 was determined by The Institute for Genomic Research (TIGR; Rockville, MD, USA) [15].In our earlier study, we demonstrated that a clinical isolate of Prevotella nigrescens is able to produce extracellular viscous material that might contribute to its biofilm formation [16]. In t
%U http://www.biomedcentral.com/1471-2180/9/11