%0 Journal Article
%T Phenotypic modulation of auto-reactive cells by insertion of tolerogenic molecules via MSC-derived exosomes
%A Aram Mokarizadeh
%A Nowruz Delirezh
%A Ahhmad Morshedi
%A Ghasem Mosayebi
%J Veterinary Research Forum
%D 2012
%I Urmia University
%X Auto-reactive cells-mediated immune responses are responsible for the current tissue damages during autoimmunity. Accordingly, functional modulation of auto-reactive cells has been a pivotal aim in many of recent studies. In the current study, we investigated the possibility for insertion of regulatory molecules onto auto-reactive cells through exosomal nano-shuttles as a novel approach for phenotype modification of auto-reactive cells. The exosomes were isolated from supernatant of mesenchymal stem cells culture. Resultant exosomes co-cultured with lymphocytes were harvested from established EAE mice in the presence of antigenic MOG35-55 peptide. After 24 hr, insertion of exosomal tolerogenic molecules (PD-L1, TGF-¦Â, galectin-1) onto auto-reactive cells were explored through flow cytometry. The potency of exosomal inserted membrane molecules to modulate phenotype of auto-reactive lymphocytes was assessed upon ELISA test for their-derived cytokines IFN-¦Ã and IL-17. Incorporation of exosomal molecules into lymohocytes¡¯ membrane was confirmed by flow cytometric analyses for surface levels of mentioned molecules. Additionally, the decreased secretion of IFN-¦Ã and IL-17 were detected in exosome pre-treated lymphocytes upon stimulation with MOG peptide. Mesenchymal stem cells -derived exosomes showed to be efficient organelles for insertion of bioactive tolerogenic molecules onto auto-reactive cells and modulation of their phenotypes.
%K Auto-reactive cell
%K EAE
%K MSC
%K Exosome
%K Tolerogenic molecule
%U www.urmia.ac.ir/vrf/Shared%20Documents/pdf/vol-3%20no-4/257-261-0242.pdf