%0 Journal Article
%T Direct and negative regulation of the sycO-ypkA-ypoJ operon by cyclic AMP receptor protein (CRP) in Yersinia pestis
%A Lingjun Zhan
%A Lei Yang
%A Lei Zhou
%A Yingli Li
%A He Gao
%A Zhaobiao Guo
%A Lianfeng Zhang
%A Chuan Qin
%A Dongsheng Zhou
%A Ruifu Yang
%J BMC Microbiology
%D 2009
%I BioMed Central
%R 10.1186/1471-2180-9-178
%X The sycO, ypkA and yopJ genes constitute a single operon in Y. pestis. CRP specifically binds to the promoter-proximate region of sycO, and represses the expression of the sycO-ypkA-yopJ operon. A single CRP-dependent promoter is employed for the sycO-ypkA-yopJ operon, but two CRP binding sites (site 1 and site 2) are detected within the promoter region. A CRP box homologue is found in site 1 other than site 2. The determination of CRP-binding sites, transcription start site and core promoter element (-10 and -35 regions) promotes us to depict the structural organization of CRP-dependent promoter, giving a map of CRP-promoter DNA interaction for sycO-ypkA-yopJ.The sycO-ypkA-yopJ operon is under the direct and negative regulation of CRP in Y. pestis. The sycO-ypkA-yopJ promoter-proximate regions are extremely conserved in Y. pestis, Y. pseudotuberculosis and Y. enterocolitica. Therefore, data presented here can be generally applied to the above three pathogenic yersiniae.Plague, caused by Yesinia pestis, is a zoonotic disease that threatened public health seriously. The three pathogenic Yersinia species, Y. pestis, Y. pseudotuberculosis, and Y. enterocolitica, share a type III secretion system (T3SS) that is composed of a secretion machinery, a set of translocation proteins, a control system, and six Yop effector proteins [1,2]. Through the T3SS, pathogenic yersiniae inject effectors into the cytosol of eukaryotic cells when docking at the surface of host cell. The injected Yops perturb the signaling cascades that activate the processes of phagocytosis, cytokine release and respiratory burst. As a result, phagocytosis is inhibited, recruitment of PMNs and monocyte-derived macrophages is reduced, and lymphocyte proliferation is prevented.The cyclic AMP receptor protein (CRP) is a global regulator that controls the transcription initiation for more than 100 bacterial genes/operons [3]. CRP is activated by cyclic AMP (cAMP), forming the cAMP-CRP complex. This complex bi
%U http://www.biomedcentral.com/1471-2180/9/178