%0 Journal Article
%T Investigations of hemadsorption characteristics of glycoprotein HN and F antigens of Newcastle Disease virus envelope in vitro
%A Ni£¿avi£¿ Jakov
%A Mili£¿ Nenad
%J Veterinarski Glasnik
%D 2006
%I Faculty of Veterinary Medicine, Belgrade
%R 10.2298/vetgl0606299n
%X The objective of these investigations was to examine the hemadsorption characteristics of glycoprotein HN and F antigens of the Newcastle Disease virus envelope. Six hours following inoculation of the cell line with the Newcastle Disease Vero virus, previously activated with 0.025 g/dl trypsin-versene, the appearance of hemadsorption was established in a smaller intensity in antigens diluted in ratios from 1:8 to 1:16. Hemadsorption was also determined in the mentioned cell lines 12 hours following inoculation in viruses diluted 1:64, while hemadsorption was present in virus dilutions of 1:256 after 24 hours. Inhibition of hemadsorption in inoculated cells with immune serum dilutions of 1:64 was established 24 hours following inoculation of the cell line with Vero samples of the Newcastle Disease virus, activated with trypsin-versene and the addition of specific immune serum.
%K Newcastle Disease virus
%K Vero cells
%K hemadsorption
%K hemadsorption inhibition
%U http://www.doiserbia.nb.rs/img/doi/0350-2457/2006/0350-24570606299N.pdf