%0 Journal Article
%T Multilocus Variable-Number Tandem-Repeat Analysis, Pulsed-Field Gel Electrophoresis, and Antimicrobial Susceptibility Patterns in Discrimination of Sporadic and Outbreak-Related Strains of Yersinia enterocolitica
%A Leila M Sihvonen
%A Susanna Toivonen
%A Kaisa Haukka
%A Markku Kuusi
%A Mikael Skurnik
%A Anja Siitonen
%J BMC Microbiology
%D 2011
%I BioMed Central
%R 10.1186/1471-2180-11-42
%X MLVA discriminated 82 sporadic YE 3-4/O:3 and 2/O:9 strains into 77 types, whereas PFGE with the restriction enzyme NotI discriminated the strains into 23 different PFGE pulsotypes. The discriminatory index for a sporadic strain was 0.862 for PFGE and 0.999 for MLVA. MLVA confirmed that a foodborne outbreak in the city of Kotka, Finland in 2003 had been caused by a multiresistant YE 4/O:3 strain that was distinctly different from those of epidemiologically unrelated strains with an identical PFGE pulsotype. The multiresistance of Y. enterocolitica strains (19% of the sporadic strains) correlated significantly (p = 0.002) with travel abroad. All of the multiresistant Y. enterocolitica strains belonged to four PFGE pulsotypes that did not contain any susceptible strains. Resistance to nalidixic acid was related to changes in codons 83 or 87 that stemmed from mutations in the gyrA gene. The conjugation experiments demonstrated that resistance to CHL, STR, and SUL was carried by a conjugative plasmid.MLVA using six loci had better discriminatory power than PFGE with the NotI enzyme. MLVA was also a less labor-intensive method than PFGE and the results were easier to analyze. The conjugation experiments demonstrated that a resistance plasmid can easily be transferred between Y. enterocolitica strains. Antimicrobial multiresistance of Y. enterocolitica strains was significantly associated with travel abroad.Yersinia enterocolitica (YE) is an enteropathogenic bacterium transmitted via food or water and may cause sporadic infections as well as foodborne outbreaks of yersiniosis [1-5]. The symptoms of yersiniosis range from mild diarrhea to severe clinical manifestations and post-infectious complications such as reactive arthritis, myocarditis, glomerulonephritis, and erythema nodosum [6]. Y. enterocolitica can be divided into six biotypes, of which biotypes 1B and 2-5 are known to be pathogenic to humans.At present, pulsed-field gel electrophoresis (PFGE) is commonly used t
%U http://www.biomedcentral.com/1471-2180/11/42