%0 Journal Article
%T Procalcitonin neutralizes bacterial LPS and reduces LPS-induced cytokine release in human peripheral blood mononuclear cells
%A Giovanni Matera
%A Angela Quirino
%A Aida Giancotti
%A Maria Concetta Pulicari
%A Linda Rametti
%A Maria Luz Rodr¨ªguez
%A Maria Carla Liberto
%A Alfredo Foc¨¤
%J BMC Microbiology
%D 2012
%I BioMed Central
%R 10.1186/1471-2180-12-68
%X PCT significantly decreased (p£¿<£¿0.05) the limulus amoebocyte lysate (LAL) assay reactivity of LPS from both Salmonella typhimurium (rough chemotype) and Escherichia coli (smooth chemotype). Subsequently, the in vitro effects of PCT on LPS-induced cytokine release were studied in human peripheral blood mononuclear cells (PBMC). When LPS was pre-incubated for 30 minutes with different concentrations of PCT, the release of interleukin-10 (IL-10) and tumor necrosis factor alpha (TNF¦Á) by PBMC decreased in a concentration-dependent manner after 24 hours for IL-10 and 4 hours for TNF¦Á. The release of monocyte chemotactic protein-1 (MCP-1) exhibited a drastic reduction at 4 hours for all the PCT concentrations assessed, whereas such decrease was concentration-dependent after 24 hours.This study provides the first evidence of the capability of PCT to directly neutralize bacterial LPS, thus leading to a reduction of its major inflammatory mediators.
%U http://www.biomedcentral.com/1471-2180/12/68/abstract