%0 Journal Article
%T Multilocus sequence typing of a global collection of Pasteurella multocida isolates from cattle and other host species demonstrates niche association
%A Emily J Hotchkiss
%A J Christopher Hodgson
%A F Alex Lainson
%A Ruth N Zadoks
%J BMC Microbiology
%D 2011
%I BioMed Central
%R 10.1186/1471-2180-11-115
%X Bovine respiratory isolates were found to be clonal (ISA 0.45) with 105/128 belonging to clonal complex 13 (CC13). HS isolates were not related to bovine respiratory isolates. Of the host species studied, the majority had their own unique sequence types (STs), with few STs being shared across host species, although there was some cross over between porcine and bovine respiratory isolates. Avian, ovine and porcine isolates showed greater levels of diversity compared to cattle respiratory isolates, despite more limited geographic origins.The homogeneity of STs of bovine respiratory P. multocida observed, and the differences between these and P. multocida subpopulations from bovine non-respiratory isolates and non-bovine hosts may indicate niche association.Pasteurella multocida is a Gram-negative bacterium that causes a wide range of clinical presentations in a wide range of host species [1]. It has been shown to cause respiratory disease in many animals, including cattle [2], sheep [3] and pigs [4,5] although it is also found in the respiratory tract of apparently healthy animals [6]. The organism also causes haemorrhagic septicaemia (HS) in bovids, mainly in South and Southeast Asia and sub-Saharan Africa [7]. In pigs P. multocida contributes to atrophic rhinitis [4] and in rabbits the organism is associated with a syndrome called "snuffles" [8]. Fowl cholera in avian species is a source of great economic losses in commercial poultry flocks and also affects wild birds [9]. In humans, P. multocida infections are mainly associated with animal bites [10,11].Historically, phenotypic methods have been used to differentiate strains and it has been shown that different serotypes are associated with different hosts and clinical presentations [12]. However the usefulness of phenotypic methods is limited due to the lack of discriminatory power and the fact that they do not reflect population structure [13]. Multilocus sequence typing (MLST) provides a standardised system of t
%U http://www.biomedcentral.com/1471-2180/11/115