%0 Journal Article
%T Deterministic separation of cancer cells from blood at 10 mL/min
%A Kevin Loutherback
%A Joseph D'Silva
%A Liyu Liu
%A Amy Wu
%J AIP Advances
%D 2012
%I AIP Publishing LLC
%R 10.1063/1.4758131
%X Circulating tumor cells (CTCs) and circulating clusters of cancer and stromal cells have been identified in the blood of patients with malignant cancer and can be used as a diagnostic for disease severity, assess the efficacy of different treatment strategies and possibly determine the eventual location of metastatic invasions for possible treatment. There is thus a critical need to isolate, propagate and characterize viable CTCs and clusters of cancer cells with their associated stroma cells. Here, we present a microfluidic device for mL/min flow rate, continuous-flow capture of viable CTCs from blood using deterministic lateral displacement (DLD) arrays. We show here that a DLD array device can isolate CTCs from blood with capture efficiency greater than 85% CTCs at volumetric flow rates of up to 10 mL/min with no effect on cell viability.
%U http://link.aip.org/link/doi/10.1063/1.4758131