%0 Journal Article
%T Identification of malassezia species
%A Kindo A
%A Sophia S
%A Kalyani J
%A Anandan S
%J Indian Journal of Medical Microbiology
%D 2004
%I Medknow Publications
%X Malassezia spp. are lipophilic unipolar yeasts recognized as commensals of skin that may be pathogenic under certain conditions. The genus Malassezia now comprises of seven species. This study was aimed at using a simple practical approach to speciate Malassezia yeasts from clinical material. Seventy skin scrapings from patients with pityriasis versicolor infection, positive in 10% potassium hydroxide (KOH), were cultured onto modified DixonĦäs agar (mDixonĦäs agar) and Sabouraud dextrose agar (SDA) and incubated at 32oC. Speciation was done on the basis of Gram stain morphology, catalase test, and utilization of Tweens. Out of 70 scrapings 48 (68.75%) showed growth on mDixonĦäs agar. The commonest isolate was M. sympodialis (28, 58%) followed by M. globosa (19, 40%) and one isolate was (2%) of M. restricta. M. sympodialis was the commonest species affecting our population and there was no isolation of M. obtusa, M. slooffiae, M. pachydermatis and M. furfur.
%K Malassezia spp.
%K pityriasis versicolor
%K lipophilic yeasts
%K tween assimilation
%U http://www.ijmm.org/article.asp?issn=0255-0857;year=2004;volume=22;issue=3;spage=179;epage=181;aulast=Kindo