%0 Journal Article
%T Comparison of rapid colorimetric method with conventional method in the isolation of mycobacterium tuberculosis
%A Oberoi A
%A Kaur H
%J Indian Journal of Medical Microbiology
%D 2004
%I Medknow Publications
%X The aim of the study was to evaluate two methods (colorimetric and conventional) for isolation of Mycobacterium tuberculosis. A total of 500 clinical specimens were processed by modified Petroff¡äs method and then inoculated into MB/BacT-240 system bottles and on LJ medium slopes. The specimens included 242 sputum, 95 gastric aspirates, 47 pleural fluids, 45 CSF, 32 urine, 18 pus, 11 bronchoalveolar lavage, 3 tissue, 2 stool, 2 lymphnode specimens, 2 synovial fluid and 1 bronchial wash specimens. The isolation rate was 16.4% by the colorimetric method and 2.2% by the conventional method. The mean detection time was 16 days and 26 days respectively. Among 36 direct smear positive samples, 63.9%(23/36) and 30%(11/36) were positive by colorimetric and conventional methods respectively. Out of 464 direct smear negative samples 12.9%(60/464) and 0.6%(3/464) were positive by colorimetric and conventional methods respectively. Therefore, colorimetric method enables rapid detection leading to early diagnosis and drug susceptibility testing.
%K Mycobacterium tuberculosis
%K colorimetric method
%K coventional method
%K mean detection time
%U http://www.ijmm.org/article.asp?issn=0255-0857;year=2004;volume=22;issue=1;spage=44;epage=46;aulast=Oberoi