%0 Journal Article
%T Towards a ˇ°Sample-In, Answer-Outˇ± Point-of-Care Platform for Nucleic Acid Extraction and Amplification: Using an HPV E6/E7 mRNA Model System
%A Anja Gulliksen
%A Helen Keegan
%A Cara Martin
%A John O'Leary
%A Lars A. Solli
%A Inger Marie Falang
%A Petter Gr nn
%A Aina Karlg rd
%A Michal M. Mielnik
%A Ib-Rune Johansen
%A Terje R. Tofteberg
%A Tobias Baier
%A Rainer Gransee
%A Klaus Drese
%A Thomas Hansen-Hagge
%A Lutz Riegger
%A Peter Koltay
%A Roland Zengerle
%A Frank Karlsen
%A Dag Ausen
%A Liv Furuberg
%J Journal of Oncology
%D 2012
%I Hindawi Publishing Corporation
%R 10.1155/2012/905024
%X The paper presents the development of a “proof-of-principle” hands-free and self-contained diagnostic platform for detection of human papillomavirus (HPV) E6/E7 mRNA in clinical specimens. The automated platform performs chip-based sample preconcentration, nucleic acid extraction, amplification, and real-time fluorescent detection with minimal user interfacing. It consists of two modular prototypes, one for sample preparation and one for amplification and detection; however, a common interface is available to facilitate later integration into one single module. Nucleic acid extracts (=28) from cervical cytology specimens extracted on the sample preparation chip were tested using the PreTect HPV-Proofer and achieved an overall detection rate for HPV across all dilutions of 50%–85.7%. A subset of 6 clinical samples extracted on the sample preparation chip module was chosen for complete validation on the NASBA chip module. For 4 of the samples, a 100% amplification for HPV 16 or 33 was obtained at the 1 : 10 dilution for microfluidic channels that filled correctly. The modules of a “sample-in, answer-out” diagnostic platform have been demonstrated from clinical sample input through sample preparation, amplification and final detection.
%U http://www.hindawi.com/journals/jo/2012/905024/