%0 Journal Article
%T Molecular detection of host cytokine expression in Helicobacter pylori infected patients via semi-quantitative RT-PCR
%A Hosseini M
%A Oghalaie A
%A Habibi G
%A Nahvijoo A
%J Indian Journal of Medical Microbiology
%D 2010
%I Medknow Publications
%X Background: Helicobacter pylori (Hp) is a bacterium recognised as a main causative agent for the development of chronic active gastritis, peptic ulcer disease, gastric adenocarcinoma and primary gastric lymphoma. Objective: Determination of the levels of IFN-¦Ã (pro-inflammatory) and IL-4 (anti inflammatory) cytokine expression as indicators of Th1 and Th2 immune responses in gastric cancer (GC) and non gastric cancer (Non GC) dyspeptic patients by gene specific RT-PCR. Materials and Methods: Biopsy specimens were collected from three groups of gastric cancer (GC=18), non ulcer dyspepsia (NUD = 38) and peptic ulcer patients (PUD=20). Total RNA was extracted and complementary DNA was synthesised. PCR amplification was performed for HPRT, IFN-¦Ã and IL-4 cytokines and the intensity of each band was measured by densitometry and normalized against HPRT expression as a house keeping gene. Results: Comparison of the results from different groups of patients indicated that IFN-¦Ã gene expression was similar in nonGC dyspeptic patients (NUD and PUD groups; 3.38 ¡À 0.57,3.43 ¡À 0.41, respectively) whereas, in GC patients, it was significantly higher than others (5.52 ¡À 0.59; P < 0.0001). On the other hand, IL-4 gene expression showed no significant difference between NUD and GC patients (2.81 ¡À 0.43,2.3 ¡À 0.12 respectively), whereas the expression rate of this cytokine was significantly higher in PUD patients (3.7 ¡À 0.1; P 0.05). Our data indicate an association between Th1 and Th2 immune responses and the development of gastric cancer and peptic ulcer disease respectively.
%K Cytokine
%K IL-4
%K IFN-¦Ã
%K immune response
%U http://www.ijmm.org/article.asp?issn=0255-0857;year=2010;volume=28;issue=1;spage=40;epage=44;aulast=Hosseini