%0 Journal Article
%T Quality of DNA extracted from saliva samples collected with the Oragene£¿ DNA self-collection kit
%A Ana Paula Nunes
%A Isabel Oliveira de Oliveira
%A Betania R Santos
%A Cristini Milech
%A Liziane Pereira Silva
%A David A Gonz¨¢lez
%A Pedro C Hallal
%A Ana MB Menezes
%A Cora L Ara¨²jo
%A Fernando C Barros
%J BMC Medical Research Methodology
%D 2012
%I BioMed Central
%R 10.1186/1471-2288-12-65
%X Saliva samples were collected with an OrageneTM DNA Self-Collection Kit from 4,110 subjects aged 14¨C15£¿years. The samples were processed in two aliquots with an 8-month interval between them. Quantitative and qualitative evaluations were carried out in 20% of the samples by spectrophotometry and genotyping. Descriptive analyses and paired t-tests were performed.The mean volume of saliva collected was 2.2£¿mL per subject, yielding on average 184.8£¿¦Ìg DNA per kit. Most samples showed a Ratio of OD differences (RAT) between 1.6 and 1.8 in the qualitative evaluation. The evaluation of DNA quality by TaqMan£¿, High Resolution Melting (HRM), and restriction fragment length polymorphism-PCR (RFLP-PCR) showed a rate of success of up to 98% of the samples. The sample store time did not reduce either the quantity or quality of DNA extracted with the Oragene kit.The study results showed that a storage period of 8£¿months at room temperature did not reduce the quality of the DNA obtained. In addition, the use of the Oragene kit during fieldwork in large population-based studies allows for DNA of high quantity and high quality.
%U http://www.biomedcentral.com/1471-2288/12/65/abstract