%0 Journal Article
%T In-vivo optical detection of cancer using chlorin e6 ¨C polyvinylpyrrolidone induced fluorescence imaging and spectroscopy
%A William WL Chin
%A Patricia SP Thong
%A Ramaswamy Bhuvaneswari
%A Khee Soo
%A Paul WS Heng
%A Malini Olivo
%J BMC Medical Imaging
%D 2009
%I BioMed Central
%R 10.1186/1471-2342-9-1
%X Fluorescence imaging was performed on MGH human bladder tumor xenografted on both the chick chorioallantoic membrane (CAM) and the murine model using a fluorescence endoscopy imaging system. In addition, fiber optic based fluorescence spectroscopy was performed on tumors and various normal organs in the same mice to validate the macroscopic images. In one patient, fluorescence imaging was performed on angiosarcoma lesions and normal skin in conjunction with fluorescence spectroscopy to validate Ce6-PVP induced fluorescence visual assessment of the lesions.Margins of tumor xenografts in the CAM model were clearly outlined under fluorescence imaging. Ce6-PVP-induced fluorescence imaging yielded a specificity of 83% on the CAM model. In mice, fluorescence intensity of Ce6-PVP was higher in bladder tumor compared to adjacent muscle and normal bladder. Clinical results confirmed that fluorescence imaging clearly captured the fluorescence of Ce6-PVP in angiosarcoma lesions and good correlation was found between fluorescence imaging and spectral measurement in the patient.Combination of Ce6-PVP induced fluorescence imaging and spectroscopy could allow for optical detection and discrimination between cancer and the surrounding normal tissues. Ce6-PVP seems to be a promising fluorophore for fluorescence diagnosis of cancer.As with most cancers, early diagnosis is critical to achieve favorable prognosis. Currently, random surveillance biopsies are the existing gold standard for the identification of lesions in pre-neoplastic conditions. However this method is prone to sampling error, time-consuming, subjective and cost-inefficient. A diagnostic method that could provide rapid, automated classification of cancer lesions would increase the efficiency and comprehensiveness of malignancy screening and surveillance procedures. A variety of optical techniques have recently been utilized for the diagnostic study of cancerous tissue. These include fluorescence spectroscopy [1], Raman
%U http://www.biomedcentral.com/1471-2342/9/1