%0 Journal Article
%T Matrix Metalloproteinases in patients with Multiple Sclerosis. Metaloproteinasas de matriz en pacientes con esclerosis m¨²ltiple
%A Rebeca A. Fern¨¢ndez Carriera
%A Lourdes Fern¨¢ndez Queija
%A Rosa F. Lara Rodr¨ªguez
%A Jos¨¦ Cabrera G¨®mez
%J MediSur
%D 2009
%I Centro Provincial de Informaci¨®n de las Ciencias M¨¦dicas en Cienfuegos
%X Fundament: The proteolitic rupture of the extracellular matrix due to metalloproteinase 2 and 9 is one of the aspects that can influence in the alteration of the permeability of the blood-brain barrier (BBB) in multiple sclerosis. Objective: To determine metalloproteinase activity with gelatinous activity in patients suffering from multiple sclerosis. Methods: the cerebrospinal fluid (CSF) samples taken from 31 patients suffering from multiple sclerosis and a control group formed by 21 patients without neurological disease. The metalloproteinase 2 and 9 activities in the cerebrospinal fluid were determined by zimogr¨¢fica technique through polyacrylamide gel electrophoresis. The bands were later analysed by their molecular weight and the relative metalloproteinase 9 activity was calculated. Total protein concentrations, albumin and immunoglobulin G (IgG), the IgG rate and the Q rate were assessed to evaluate the IgG intrathecal and the functional state of the blood-brain barrier. Results: metalloproteinase 2 activity was detected in the cerebrospinal fluid of all patients and control group. Metalloproteinase 9 activity was only found in the 61.3 % of the patients. The presence of relative metalloproteinase 9 activity was neither associated with the clinical variables nor the laboratory ones. An association was found between its presence and the oligoclonal bands in patients with multiple sclerosis. In those patients under immunomodular treatment it was presented with less frequency. Conclusions: There is a possible participation of Metalloproteinase 9 in the immunopathological mechanisms of the multiple sclerosis. Fundamento: La ruptura proteol¨ªtica de la matriz extracelular por las metaloproteinasas 2 y 9 es uno de los aspectos que puede influir en la alteraci¨®n de la permeabilidad de la barrera hematoencef¨¢lica en la esclerosis m¨²ltiple. Objetivo: Determinar la actividad de las metaloproteinasas con actividad gelatinasa en pacientes con esclerosis m¨²ltiple. M¨¦todos: Las muestras de l¨ªquido cefalorraqu¨ªdeo se obtuvieron de 31 pacientes con esclerosis m¨²ltiple y un grupo control conformado por 21 pacientes sin enfermedad neurol¨®gica. Las actividades de las metaloproteinasas 2 y 9 en el l¨ªquido cefalorraqu¨ªdeo se determinaron mediante t¨¦cnica zimogr¨¢fica por electroforesis en gel de poliacrilamida, despu¨¦s las bandas fueron identificadas por sus pesos moleculares y se calcul¨® la actividad relativa de la metaloproteinasa 9. Se evaluaron las concentraciones de prote¨ªnas totales, alb¨²mina e IgG, el ¨ªndice IgG y el ¨ªndice Q para evaluar s¨ªntesis intratecal de
%K Esclerosis m¨²ltiple
%K Metaloproteinasa 2 de la matriz
%K Metaloproteinasa 9 de la matriz
%K L¨ªquido cefalorraqu¨ªdeo
%K Electroforesis
%U http://www.medisur.sld.cu/index.php/medisur/article/view/254