%0 Journal Article
%T Anal infections with concomitant Chlamydia trachomatis genotypes among men who have sex with men in Amsterdam, the Netherlands
%A Koen D Quint
%A Reinier JM Bom
%A Wim GV Quint
%A Sylvia M Bruisten
%A Maarten van der Loeff
%A Servaas A Morr¨¦
%A Henry JC de Vries
%J BMC Infectious Diseases
%D 2011
%I BioMed Central
%R 10.1186/1471-2334-11-63
%X A total of 201 Ct positive rectal swabs from MSM were selected, which were previously diagnosed as either LGV (n = 99) or non-LGV Ct infection (n = 102) according to the algorithm of Ct detection by the commercially available Aptima Combo 2 assay followed by an in-house pmpH LGV PCR. The samples were retested with the commercially available Ct-DT RHA, which differentiates between 14 major genotypes and is able to detect concomitant Ct genotypes.Excellent genotyping agreement was observed between the Ct-DT RHA and the pmpH LGV PCR (Kappa = 0.900, 95%CI = 0.845-0.955, McNemar's p = 1.000). A concomitant non-LGV genotype was detected in 6/99 (6.1%) LGV samples. No additional LGV infections were observed with the Ct-DT RHA among the non-LGV Ct group. In the non-LGV group genotype G/Ga (34.3%) was seen most frequent, followed by genotype D/Da (22.5%) and genotype J (13.7%). All LGV infections were caused by genotype L2.Concomitant non-LGV genotypes do not lead to missed LGV proctitis diagnosis. The pmpH LGV PCR displayed excellent agreement with the commercially available Ct-DT genotyping RHA test. The genotypes G/Ga, D/Da and J were the most frequent non-LGV Ct strains in MSM.Chlamydia trachomatis (Ct) is the most common sexually transmitted bacterial disease worldwide. A Ct infection can infect different mucosal linings, with the majority of cases in the urogenital tract but also the rectum, oropharynx or conjunctiva.In men who have sex with men (MSM), the rectum is often the only infected site, without a concurrent Ct infection in the urogenital tract [1]. Like urogenital infections, most rectal Ct infections remain asymptomatic [2]. Nevertheless, an asymptomatic rectal Ct infection can contribute to HIV transmission due to mucosal damage and recruitment of dendritic cells [3].In general, Ct infections caused by non-LGV Ct genotypes (D-K) give few or no symptoms since they remain confined to the mucosal lining and do not trigger overt immunological reactions [4]. Ct i
%U http://www.biomedcentral.com/1471-2334/11/63