%0 Journal Article
%T Characterization of lymphocyte subsets over a 24-hour period in Pineal-Associated Lymphoid Tissue (PALT) in the chicken
%A Jeffrey A Mosenson
%A John A McNulty
%J BMC Immunology
%D 2006
%I BioMed Central
%R 10.1186/1471-2172-7-1
%X PALT comprised approximately 10% of the total pineal area. Image analysis of immunocytochemically stained sections showed that the majority of lymphocytes were CD3+ (80%) with the remaining 20% comprising B-cells and monocytes (Bu-1+), which tended to distribute along the periphery of the PALT. T-cell subsets in PALT included CD4+ (75¨C80%), CD8+ (20¨C25%), TCR¦Á¦Â/V¦Â1+ (60%), and TCR¦Ã¦Ä+ (15%). All of the T-cell phenotypes were commonly found within the interfollicular septa and follicles of the pineal gland. However, the ratios of CD8+/CD4+ and TCR¦Ã¦Ä+/TCR¦Á¦Â/V¦Â1+ within the pineal tissue were each 1:1, in contrast to the PALT where the ratios of CD8+/CD4+ and TCR¦Ã¦Ä+/TCR¦Á¦Â/V¦Â1+ each approximated 1:4. Bu-1+ cells were only rarely seen in the pineal interstitial spaces, but ramified Bu-1+ microglia/macrophages were common in the pineal follicles. Effects of the 24-h light:dark cycle on these lymphocyte-pineal interactions were suggested by an increase in the area of PALT, a decline in the density of TCR¦Á¦Â/V¦Â1+ cells, and a decline in the area density of Bu-1+ microglia at the light:dark interphase (1900 h) compared to the dark:light interphase (0700 h).The degree of lymphocyte infiltration in the pineal suggests novel mechanisms of neuro-immune interactions in this part of the brain. Our results further suggest that these interactions have a temporal component related to the 24-hour light:dark cycle and that CD8+ and TCR¦Ã¦Ä+ T-cells are preferentially recruited to the pineal follicles. Pineal microglia/macrophages were common and represent an important candidate for mediating these lymphocyte-pineal interactions via secretion of cytokines and chemokines.The view that the central nervous system (CNS) is an immunologically privileged site has been widely accepted based on classic experiments such as those of Medawar [1], who showed that rabbit skin allografted into the CNS failed rejection. This immunologic privilege is specifically related to the low levels of MHC class II e
%U http://www.biomedcentral.com/1471-2172/7/1