%0 Journal Article
%T The ¦ÊB transcriptional enhancer motif and signal sequences of V(D)J recombination are targets for the zinc finger protein HIVEP3/KRC: a site selection amplification binding study
%A Carl E Allen
%A Chi-ho Mak
%A Lai-Chu Wu
%J BMC Immunology
%D 2002
%I BioMed Central
%R 10.1186/1471-2172-3-10
%X Both fusion proteins selected sequences that were similar to the ¦ÊB motif or the canonical elements of the V(D)J recombination signal sequences (RSS) from a pool of degenerate oligonucleotides. Specifically, the ZAS-N domain selected sequences similar to the canonical RSS nonamer, while ZAS-C domain selected sequences similar to the canonical RSS heptamer. In addition, both KRC fusion proteins selected oligonucleoties with sequences identical to heptamer and nonamer sequences within endogenous RSS.The RSS are cis-acting DNA motifs which are essential for V(D)J recombination of antigen receptor genes. Due to its specific binding affinity for RSS and ¦ÊB-like transcription enhancer motifs, we hypothesize that KRC may be involved in the regulation of V(D)J recombination.The ZAS gene family is an emerging family of important transcriptional proteins that have been implicated in the regulation of gene expression of the HIV-1 long terminal repeat [1], and genes encoding ¦ÁA-crystallin [2], somatostatin receptor type II [3], the small calcium binding protein S100A4/mts1 [4], and type II collagen [5] via specific promoter or enhancer elements. Three human genes, HIVEP1/Mbp1/PRDII-BF1 [6-9], HIVEP2/Mbp2 [10-12], and HIVEP3 [13], and their respective mouse counterparts ¦ÁACRYBP1 [2], MIBP1 [3], and KRC [14,15], as well as rat AGIE-BP1/MIBP1 [16,17] have been cloned and characterized. In addition, a distant relative Schnurri (Shn) has been identified in Drosophila [18-20]. Although little is known about the physiological functions of the mammalian ZAS proteins, Shn has been shown to be an important transcription regulator during embryonic development. Shn modulates transcription by relieving the repression of the nuclear protein Brinker and, in association with SMAD, mediates transcription response of the decapentaplegic pathway [21,22].Each ZAS gene encodes large sequence-specific DNA-binding proteins with Mr >250,000 that contain two widely separated of C2H2-type zinc finger pa
%U http://www.biomedcentral.com/1471-2172/3/10