%0 Journal Article
%T Genotypes of vitamin D and estrogen receptors in pre and perimenopausal women from C車rdoba, Argentina Genotipos de los receptores de vitamina D y de estr車geno en mujeres pre y perimenop芍usicas de C車rdoba, Argentina
%A Mar赤a Ulla
%A Adriana P谷rez
%A Vanina El赤as
%A Miriam Binci
%J Medicina (Buenos Aires)
%D 2007
%I Fundaci車n Revista Medicina
%X The aim of this study was to determine the frequency of vitamin D receptor and estrogen receptor genotypes and their relationship with the lumbar spine or femoral neck bone mineral density in healthy pre and perimenopausal women from C車rdoba (Argentina) and adjacent areas. Genotypes were assessed by restriction fragment length polymorphism-polymerase chain reaction technique. Bsm I and Fok I for vitamin D receptor gene and XbaI and PvuII for estrogen receptor gene were used as restrictases. Two hundred and ten healthy pre and perimenopausal women were recruited and analyzed by age. Calcemia and serum parathyroid hormone did not change, but serum P and b-CrossLaps decreased with age. Femoral neck bone mineral density decreased significantly after 30 years old. Vitamin D receptor and estrogen receptor genotype frequencies were similar to those from other Caucasian women. No association between vitamin D receptor and estrogen receptor genotypes with the lumbar spine or femoral neck bone mineral density has been detected. Analysis of interaction between vitamin D receptor and estrogen receptor genes using covariates such as age, height and body mass index did not show any influence of the combination of those genotypes on bone mineral density. Lifestyle, smoking and alcohol intake had no effect on lumbar spine and femoral neck bone mineral density. To conclude, these data do not support the hypothesis that vitamin D receptor and estrogen receptor genotypes influence on lumbar spine and femoral neck bone mineral density in healthy pre and perimenopausal women from this area of Argentina. El prop車sito del estudio fue determinar la frecuencia de los genotipos de los receptores de vitamina D y de estr車geno y su relaci車n con la densidad mineral 車sea en mujeres sanas pre y perimenop芍usicas de la ciudad de C車rdoba y alrededores. Los genotipos se determinaron con la t谷cnica de reacci車n en cadena de la polimerasa y an芍lisis de los polimorfismos de longitud de fragmentos de restricci車n. Se usaron como restrictasas Bsm I y Fok I para el gen del receptor de vitamina D y Pvu II y Xba I para el gen del receptor de estr車geno. Se reclutaron y agruparon por edad doscientos diez mujeres pre y peri-menop芍usicas. Sus niveles s谷ricos de Ca y de hormona paratiroidea fueron similares, pero los de f車sforo y b-Cross Laps disminuyeron con la edad. La densidad mineral 車sea de cuello femoral disminuy車 despu谷s de los 30 a os. Las frecuencias genot赤picas de ambos receptores fueron similares a aqu谷llas de otras mujeres cauc芍sicas. No hubo asociaci車n entre los genotipos de los receptores
%K VDR
%K ER
%K Densidad mineral 車sea
%K Mujeres pre y perimenop芍usicas
%K VDR
%K ER
%K Bone mineral density
%K Pre and perimenopausal women
%U http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0025-76802007000100005