%0 Journal Article
%T Increased production of pro-inflammatory cytokines and enhanced T cell responses after activation of human dendritic cells with IL-1 and CD40 ligand
%A Amy Wesa
%A Anne Galy
%J BMC Immunology
%D 2002
%I BioMed Central
%R 10.1186/1471-2172-3-14
%X Human DC co-activated in vitro by CD40L and IL-1¦Â expressed numerous cytokine genes including IL-12¦Â, IL-23 p19, IL-1¦Â, IL-1¦Á, IL-1Ra, IL-10, IL-6, IL-18 and IFN-¦Ã. These DC produced high levels of IL-12 protein and appeared capable of producing IFN-¦Ã. Potent CD4+ and CD8+ T cell-stimulatory properties were acquired by DC under conditions that also induced IL-12. Notably, these DC induced rapid differentiation of fluMP-specific CD8+ T cells. Molecules related to IL-1¦Â, like IL-1¦Á, co-induced IL-12 secretion whereas IL-18 did not. Conversely, the inhibitor IL-1Ra, produced endogenously by DC curtailed IL-12 production in response to CD40L.IL-1 and IL-1Ra play a biologically-relevant role in the positive and negative regulation of DC activation. In conjunction with CD40L, IL-1 sends a powerful activation signal to DC that could be distinguished from other modes of activation. This signal enables the production of pro-inflammatory cytokines by DC, and enhances the differentiation of na£¿ve T cells into effectors of type-1 cellular immune responses.Dendritic cells (DC) are highly effective antigen presenting cells (APCs) capable of stimulating the differentiation of na£¿ve lymphocytes into effector cells (reviewed in [1]). Specific properties of the DC determine the quality of immune responses that they initiate. These properties are determined by complex and dynamic changes both in the DC and in its environment. Notably, DC undergo a so-called process of maturation that changes migratory properties, diminishes antigen uptake, enhances antigen processing and presentation and induces the production of an array of cytokines (reviewed in [2]). The qualitative, quantitative and temporal regulation of cytokines produced by DC is thought to be a determining factor in the development of immune responses initiated by these APCs but molecules and mechanisms that regulate such process are not entirely understood.Activated T cells provide strong stimuli that activate cytokine produc
%K IL-1
%K CD40L
%K IL-12
%K IL-23
%K dendritic cells
%K human
%U http://www.biomedcentral.com/1471-2172/3/14