%0 Journal Article
%T Aktywno   przeciwgrzybicza nowych, syntetycznych analog¨®w allicyny - badania wst pne
%A Urszula Nawrot
%A Ewa Zaczy¨˝ska
%A Anna Czarny
%A Vira Lubenets
%J Medical Mycology
%D 2011
%I 
%X Introduction: Despite of the great progress made in antifungal therapy, there is still a need in the development of efficient and safe antimycotics, which could be used to control fungal grow in infected mucosa and deep-seated tissues. Development of phyto-preparations ¨C highly effective, non-toxic and without side effects, is an urgent task. Thiosulfinates, garlic compounds characterized by the ability to inhibit the growth of different microorganisms. The main active substance of garlic is allicin, one of the esters of thiosulfinic acid. Aim of the study: The purpose of present study was to test in vitro the cytotoxicity and antimycotic activity of two new synthesized thiosulfonates and rhamnolipid biosurfactant (RL) isolated from Pseudomonas sp. PS-17. Material and methods: Cytotoxicity of new synthetic tiosulfonates and rhamnolipid was determined in vitro on human lung cell line A549 and mouse fibroblast cells L929. Antifungal activity was tested on Candida albicans ATCC 90028, Candida glabrata ATCC 90030 and Aspergillus fumigatus IHEM 13934 according to M-27-A3 (CLSI, USA) microdilution method. Results: Thiosulfonates and rhamnolipid were characterized by low level of cytotoxicity. Thiosulfonates showed inhibitory activity against Candida and Aspergillus, however A. fumigatus seems to be more susceptible than Candida. Rhamnolipid RL displayed no activity against C. albicans as well as A. fumigatus, in tested range of concentrations. Conclusion: The low concentrations of synthetic tiosulfonates inhibited growth C. albicans, C. glabrata and A. fumigatus. No antymicotic activity of rhamnolipid in 100 ¦Ěg ml-1 was observed.
%K dzia anie przeciwgrzybicze
%K ramnolipidy
%K tiosulfonaty syntetyczne
%K 
%U http://cornetis.pl/pliki/ML/2011/4/ML_2011_4_197.pdf