%0 Journal Article
%T Increased susceptibility of 129SvEvBrd mice to IgE-Mast cell mediated anaphylaxis
%A Muthuvel Arumugam
%A Richard Ahrens
%A Heather Osterfeld
%A Leah C Kottyan
%A Xun Shang
%A John A Maclennan
%A Nives Zimmermann
%A Yi Zheng
%A Fred D Finkelman
%A Simon P Hogan
%J BMC Immunology
%D 2011
%I BioMed Central
%R 10.1186/1471-2172-12-14
%X 129S5 mice had significantly increased susceptibility to passive and oral OVA-induced active anaphylaxis. Increased susceptibility to anaphylaxis was associated with increased homeostatic mast cell levels but not OVA-specific IgE or IgG1 levels. In vitro analyses of BMMCs revealed no difference in Fc¦ÅRI and c-Kit expression, however, 129S5 BMMCs possessed greater proliferative capacity and reduced caspase-3-mediated apoptosis. IgE-BMMC degranulation assays demonstrated no difference in degranulation efficiency. Furthermore, 129S5 mice possessed increased sensitivity to histamine-induced hypothermia.We conclude that 129S5 mice have increased susceptibility to anaphylaxis as compared to BALB/c strain and their increased susceptibility was associated with altered mast cell proliferation and homeostatic tissue levels and responsiveness to histamine. Given the wide spread usage of the 129SvEvBrd strain of mice in experimental gene targeting methodology, these data have important implications for studying IgE-reactions in mouse systems.Anaphylaxis is a rapid, life threatening allergic reaction often triggered by food, drugs, insect venoms, latex, or allergen immunotherapy [1-5]. Early clinical and experimental evidence suggests that systemic anaphylaxis is mediated by IgE/mast cell degranulation and the rapid release of preformed mediators, including histamine, proteoglycans, PAF, serotonin, tryptase, chymase and lipid-derived mediators (PGD2 and LTC4, LTD4 and LTE4) [6,7]. These mediators are reportedly acting on target cells to induce vasodilation, increased vascular permeability, hypotension, bronchospasm and, as a result, shock.With the recent emergence of several gene knockout mice (Fc£¿RI, histamine decarboxylase, IL-4R¦Á) there has been significant focus on employment of animal models of IgE-mediated passive and active systemic and oral antigen-induced anaphylaxis in an attempt to decipher the relative contributions of inflammatory cells and cytokines to disease path
%U http://www.biomedcentral.com/1471-2172/12/14